I think I found the answer in the documentation on The Configuration Window.
(4) - Run a .exe in this virtual drive
"Allows you to run an .exe or .msi file within the context of the virtual drive or program highlighted. Will use whatever WineBuild is attached to that particular virtual drive, as well as sandboxing any action that happens therein."
Linux Mint Cinnamon 19.3 Tricia
PlayonLinux v4.3.4
Wine 5.0